Isaac married Mary Sparhawk on 26 Apr 1753 in Brookline, Norfolk, Massachusetts, USA. Mary (daughter of Thomas Sparhawk and Mary Oliver) was born on 3 Jan 1731 in Cambridge, Middlesex, Massachusetts, USA; was christened on 30 Sep 1733 in Cambridge, Middlesex, Massachusetts, USA; died on 26 Dec 1778; was buried in Walnut Street Cemetery, Brookline, Norfolk, Massachusetts, USA. [Group Sheet] [Family Chart]

1. Susanna Gardner was born on 28 Feb 1754; died on 2 Jun 1814; was buried in Walnut Street Cemetery, Brookline, Norfolk, Massachusetts, USA.
2. Mary Gardner was born on 15 Apr 1756 in Brookline, Norfolk, Massachusetts, USA; died on 31 Mar 1809; was buried in Old Burying Ground,Littleton,Middlesex, Massachusetts, USA.
3. General Isaac Sparhawk Gardner, III was christened on 26 Nov 1758 in Watertown, Middlesex, Massachusetts, USA; died on 6 Dec 1818; was buried in Walnut Street Cemetery, Brookline, Norfolk, Massachusetts, USA.
4. Sibyl Gardner was born on 18 Jul 1760; died on 20 Feb 1802.
5. Colonel William Gardner was born on 3 Aug 1762 in Brookline, Norfolk, Massachusetts, USA; died on 5 Aug 1832 in Avon Springs, Livingston,New York, USA; was buried in Christ Church Cemetery,Manilus,Onondaga,New York, USA.
6. Elizabeth Gardner was born on 22 Jan 1764; died on 28 Nov 1777.
7. Thomas Gardner was born on 3 Oct 1765 in Brookline, Norfolk, Massachusetts, USA; died on 24 Nov 1833.
8. Abigail Gardner was born in Oct 1767 in Brookline, Norfolk, Massachusetts, USA; died in Mar 1843.
9. John Gardner was born on 4 Oct 1769; died on 30 Nov 1769.
10. Hannah Gardner was born on 25 Jan 1771 in Massachusetts, USA; died about 1860.
11. Samuel Gardner was born about 1772.